rabbit polyclonal anti mouse cd19 antibody  (Cell Signaling Technology Inc)

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 7-marker panel for human FFPE tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Concentration Assay, Incubation

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 6-marker panel for formalin free zinc salt-fixed tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Incubation

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 7-marker TLS panel on human prostate cancer FFPE tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human melanoma tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human prostate cancer tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 6-marker TLS panel on murine gastric cancer tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in murine gastric cancer tissue sections using the 6-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at a 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Polymer, Plasmid Preparation, Recombinant, Saline, Software, Staining, Imaging

Journal: The American Journal of Pathology

Article Title: Dermatan Sulfate Interacts with Dead Cells and Regulates CD5 + B-Cell Fate

doi: 10.1016/j.ajpath.2011.01.028

Figure Lengend Snippet: Association of DS with apoptotic or dead cells. A: Uptake of DS-Cy5 by apoptotic or dead cells. Mouse spleen cells were cultured with DS-Cy5 for 1 day and stained with PI. Gated dead and live cells in the forward/side scatter (FSC/SSC) plot (left) are colored in red and green, respectively. Only apoptotic or dead cells in the culture are associated with DS uptake (right). Apoptotic (PI+) cells appear to associate with slightly more DS than dead (PI++) cells. B: Binding of apoptotic or dead cells (red) to DS. Cells were cultured with DS for 3 days and stained with anti-CD19-PE and DS-Cy5. Note that all dead cells bind DS-Cy5 irrespective of CD19 expression. C: Association of DS with small apoptotic/dead cell bodies. Cultured mouse spleen cells were stained with DS-AF568 (red) and DAPI (blue). D: Immuno-gold electron microscopy demonstrating association of DS (small dots) with apoptotic bodies in a fragmented mouse spleen cell. Mouse spleen cells were cultured with DSbt for 1 day and stained with gold-labeled anti-biotin antibodies. The cell shown is fragmented into two condensed nuclear fragments (condensed areas on lower left and lower right), one granular area, and an autophagosome (upper right). Note that DS is mostly associated with the granular material and also with the nuclear membrane. Scale bar = 100 nm.

Article Snippet: CD19 was detected by incubating with polyclonal rabbit anti-mouse CD19 IgG (Santa Cruz Biotech, Santa Cruz, CA) at 25°C for 1 hour, followed by goat anti-rabbit IgG horseradish peroxidase (Santa Cruz Biotech) in blocking buffer at 25°C for 1 hour.

Techniques: Cell Culture, Staining, Binding Assay, Expressing, Electron Microscopy, Labeling, Membrane

Journal: The American Journal of Pathology

Article Title: Dermatan Sulfate Interacts with Dead Cells and Regulates CD5 + B-Cell Fate

doi: 10.1016/j.ajpath.2011.01.028

Figure Lengend Snippet: DS stimulates CD5+ B-cell proliferation in cell culture. A: Mouse spleen cells were cultured with DS or medium alone (control) for 1 to 4 days. Dot plots of 10,000 total (ungated) events are shown in each graph. Note the progressive expansion of CD5+CD19+ cells (red circles) in DS but not in medium alone (numbers indicate percentage of events in red circles relative to total events). B: DS and LPS stimulate different B-cell populations, as demonstrated by FACS analysis of murine spleen cells cultured with medium alone, DS, or LPS for 4 days. Density plots of 20,000 total events are shown in each graph and each labeled with the percentage of live B cells relative to total cells in the analyzed culture. Note that the CD5 levels of CD5+CD19+ B-1a cells stimulated by DS are characteristically lower than those of T (CD5+CD19−) cells. LPS stimulates CD5+CD19− B-2 cells. Cells with low CD19 levels (left lower quadrant) are dead B cells. Antibodies: anti-CD5-PE and anti-CD19-PE-Cy5. FSC, forward scatter.

Article Snippet: CD19 was detected by incubating with polyclonal rabbit anti-mouse CD19 IgG (Santa Cruz Biotech, Santa Cruz, CA) at 25°C for 1 hour, followed by goat anti-rabbit IgG horseradish peroxidase (Santa Cruz Biotech) in blocking buffer at 25°C for 1 hour.

Techniques: Cell Culture, Control, Labeling

Journal: The American Journal of Pathology

Article Title: Dermatan Sulfate Interacts with Dead Cells and Regulates CD5 + B-Cell Fate

doi: 10.1016/j.ajpath.2011.01.028

Figure Lengend Snippet: Involvement of CD19 and CD5 in DS-mediated stimulation of B-1a cells. A: Uptake of labeled DS by CD5+CD19+ cells in mouse spleen cell culture. Only CD5+CD19+ cells display fluorescence (DS-AF568, false-colored in white). Cells positive for either CD5 (labeled with AF647, red) only or CD19 (labeled with AF488, green) only did not incorporate DS-AF568. Nuclei were stained with DAPI (blue). B: Colocalization of DS and CD19 demonstrated by staining DS-AF568–cultured mouse splenocytes with anti-CD19-FITC and DAPI. The example demonstrates partial colocalization (yellow) of DS (red) and CD19 (green) in an activated B cell. C: Colocalization of DS and CD5 demonstrated by staining DS-AF568–cultured mouse splenocytes with anti-CD5-FITC and DAPI. DS shows partial colocalization with CD5 (yellow). D: Western blot detection of CD19 (left) in total unfractionated protein extract and a DS-binding fraction (0.4 mol/L NaCl). Western blot detection of CD5 (right) in total unfractioned protein extract and both nonbinding (0.2 mol/L NaCl) and DS-binding (0.4 mol/L NaCl) fractions. Numbers above lanes denote molar concentrations of NaCl used for elution. E: DS-mediated expansion of CD5+CD19−IgM+ B cells from CD19-deficient mice (middle). Spleen cells from C.Cg-Cd19tm1(cre)CgnIghb/J mice were cultured with DS for 4 days. Dot plots show 10,000 total events each. Cells in the red circles are negative for CD19 and positive for IgM. Cultures with medium only or LPS (left and right) are shown as controls. Antibody: anti-CD5-PE. FSC, forward scatter.

Article Snippet: CD19 was detected by incubating with polyclonal rabbit anti-mouse CD19 IgG (Santa Cruz Biotech, Santa Cruz, CA) at 25°C for 1 hour, followed by goat anti-rabbit IgG horseradish peroxidase (Santa Cruz Biotech) in blocking buffer at 25°C for 1 hour.

Techniques: Labeling, Cell Culture, Fluorescence, Staining, Western Blot, Binding Assay